So far, more than 1,000 user accounts have been frozen for periods of time ranging from five to 90 days.
The rules went into effect on Wednesday on Didi’s bike-rental platforms Qingju and Bluegogo.
Didi’s struggles in the bike-rental market led to a launch of its own brand, Qingju.
The news of ofo’s E2-2 round comes amid rumors of acquisition by Didi Chuxing.
Cash-strapped Ofo could try wheeling its business down another path.