Beijing and Shanghai have given no indication of whether the bans will be lifted.
Discarded bikes are languishing after the rental-bike boom went bust due to high retrieval costs.
Didi hopes new strategies will help it complete a U-turn that will get it out of the dead-end in which it finds itself.
So far, more than 1,000 user accounts have been frozen for periods of time ranging from five to 90 days.
The rules went into effect on Wednesday on Didi’s bike-rental platforms Qingju and Bluegogo.
Didi’s struggles in the bike-rental market led to a launch of its own brand, Qingju.
While Ofo’s legal troubles pile up, millions of users are reportedly still waiting for their deposits.
A moral in Ofo’s rise and fall: ‘It is dangerous to violate common sense.’